Human OX40/TNFRSF4 ELISA Kit (Colorimetric)

ELISA: Human OX40/TNFRSF4 ELISA Kit (Colorimetric) [NBP2-22043]

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 78 - 5,000 pg/mL
• Sensitivity: 1.8 pg/mL

Human OX40/TNFRSF4 ELISA Kit (Colorimetric) Summary

• Description: The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.
• Specificity: The assay detects both natural and recombinant human OX40/TNFRSF4. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human OX40/TNFRSF4 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 78 - 5,000 pg/mL
• Sensitivity: 1.8 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 12.0%
• Intra-Assay: CV% < 8.0%
• Spike Recovery: 88%
• Sample Volume: 25 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human OX40/TNFRSF4 ELISA Kit (Colorimetric)

• ACT-135
• ACT35 antigen
• ACT35ATC35 antigen
• CD134 antigen
• CD134
• Ly-70
• OX40 cell surface antigen
• OX40 homologue
• OX40
• OX40L receptor
• OX40lymphoid activation antigene ACT35
• TAX transcriptionally-activated glycoprotein 1 receptor
• tax-transcriptionally activated glycoprotein 1 receptor
• TNFRSF4
• tumor necrosis factor receptor superfamily member 4
• tumor necrosis factor receptor superfamily, member 4
• Txgp1
• TXGP1L
• TXGP1LOX40 antigen

Background

The human sCD134/OX40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD134/OX40.

OX40 (CD134) is a member of the tumor necrosis (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T-cells. Interaction of OX40 with its ligand, OX40L, is thought to be important in T cell activation through T cell/antigen-presenting cell (APC) interaction. Ligation of OX40 induces clonal expansion and survival of CD4 cells during primary responses, and results in the accumulation of greater numbers of memory cells with time.
A soluble isoform of OX40 has been described. Measurement of this molecule may have diagnostic value in polymyositis and granulomatous myopathy, in T cell lymphoma and lymphomatoid papulosis, in proliferative lupus nephritis, in rheumatoid arthritis, in HIV infection, in viral infections of the lung, in the regulation of graft-versus-host disease,in myocarditis and dilated cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for OX40/TNFRSF4 ELISA Kit (NBP2-22043). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFRSF4