OX40/TNFRSF4 Antibody [Phycoerythrin]

Activated-mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse OX40/TNFRSF4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1256P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Phycoerythrin

OX40/TNFRSF4 Antibody [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OX40/TNFRSF4
  Val20-Pro211
  Accession # P47741
• Specificity: Detects mouse OX40/TNFRSF4 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse (rm) EDAR, rm4-BB, rmCD27, rmDR3, rmGITR, rmNGF R, rmCD30, rmCD40, rmFas, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OX40/TNFRSF4 Antibody [Phycoerythrin]

• ACT-135
• ACT35 antigen
• ACT35ATC35 antigen
• CD134 antigen
• CD134
• Ly-70
• OX40 cell surface antigen
• OX40 homologue
• OX40
• OX40L receptor
• OX40lymphoid activation antigene ACT35
• TAX transcriptionally-activated glycoprotein 1 receptor
• tax-transcriptionally activated glycoprotein 1 receptor
• TNFRSF4
• tumor necrosis factor receptor superfamily member 4
• tumor necrosis factor receptor superfamily, member 4
• Txgp1
• TXGP1L
• TXGP1LOX40 antigen

Background

OX40, also known as CD134, was originally identified as an activated rat CD4⁺ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4⁺ T cells, but is also expressed on activated human and mouse CD8⁺ T cells. The ligand of OX40 is OX40 Ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).

1. Paterson, D.J. et al. (1987) Mol. Immunol. 24:1281.
2. Mallett, S. et al. (1990) EMBO J. 9:1063.
3. Calderhead, D.M. et al. (1993) J. Immunol. 151:5261.
4. Baum, P.R. et al. (1994) EMBO J. 13:3992.
5. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
6. Bansal-Pakala, P. et al. (2001) Nature Medicine 7:907.
7. Rogers, P.R. et al. (2001) Immunity 15:445.
8. Taylor, L. and H.J. Schwarz (2001) Immunol. Methods 255:67.
9. Weinberg, A.D. (2002) Trends in Immunol. 23:102.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OX40/TNFRSF4 Antibody (FAB1256P)(4)

Publications using FAB1256P

• Walter Jackson Iii, ;Yang, Y;Salman, S;Dordai, D;Lyu, Y;Datan, E;Drehmer, D;Huang, TY;Hwang, Y;Semenza, GL; Pharmacologic HIF stabilization activates costimulatory receptor expression to increase antitumor efficacy of adoptive T cell therapy Science advances 2024-08-30 [PMID: 39196939] (Flow Cytometry, Transgenic Mouse)
• Montes de Oca R, Alavi AS, Vitali N et al. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo Molecular Cancer Therapeutics 2021-10-01 [PMID: 34253590]
• Montes de Oca R, Alavi AS, Vitali N et al. Belantamab Mafodotin (GSK2857916) Drives Immunogenic Cell Death and Immune-mediated Antitumor Responses In Vivo Molecular Cancer Therapeutics 10/1/2021 [PMID: 34253590]
• W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018-01-18;0(0):. 2018-01-18 [PMID: 29344664] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TNFRSF4
• Uniprot:
  • P47741()