OX40/TNFRSF4 Antibody (AP-MAB0833) - Azide and BSA Free

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready, Func
• Clone: AP-MAB0833
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

OX40/TNFRSF4 Antibody (AP-MAB0833) - Azide and BSA Free Summary

• Immunogen: Recombinant mouse OX-40 protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFRSF4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • T Cell Stimulation
• Application Notes: Flow Cytometry, Immunohistochemistry-Frozen, Stimulation of T cell proliferation and does not blocking the binding of CD134(OX-40) to its ligand. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for OX40/TNFRSF4 Antibody (AP-MAB0833) - Azide and BSA Free

• ACT-135
• ACT35 antigen
• ACT35ATC35 antigen
• CD134 antigen
• CD134
• Ly-70
• OX40 cell surface antigen
• OX40 homologue
• OX40
• OX40L receptor
• OX40lymphoid activation antigene ACT35
• TAX transcriptionally-activated glycoprotein 1 receptor
• tax-transcriptionally activated glycoprotein 1 receptor
• TNFRSF4
• tumor necrosis factor receptor superfamily member 4
• tumor necrosis factor receptor superfamily, member 4
• Txgp1
• TXGP1L
• TXGP1LOX40 antigen

Background

CD134 is a type I integral membrane protein, also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases. This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with Recombinant mouse OX-40 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OX40/TNFRSF4 Antibody (NBP1-06681)(1)

Publication using NBP1-06681

• Ma Y, Li J, Wang H et al. Combination of PD1 Inhibitor and OX40 Agonist Induces Tumor Rejection and Immune Memory in Mouse Models of Pancreatic Cancer Gastroenterology 2020-03-13 [PMID: 32179091] (Mouse)

Bioinformatics

• Gene Symbol: TNFRSF4
• Entrez:
  • 22163(Mouse)