OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clone: MM0505-8S23
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free Summary

• Immunogen: Human recombinant OX40 Ligand/TNFSF4
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • CyTOF-ready
  • Flow Cytometry 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-200
  • Neutralization 50 - 200 molar excess
  • Western Blot 1:1000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free

• CD134 ligand
• CD134L
• CD252 antigen
• CD252
• Glycoprotein Gp34
• gp34
• OX40 antigen ligand
• OX40 Ligand
• OX40L
• OX-40L
• OX4OL
• TAX transcriptionally-activated glycoprotein 1
• tax-transcriptionally activated glycoprotein 1 (34kD)
• tax-transcriptionally activated glycoprotein 1, 34kD
• TNFSF4
• tumor necrosis factor (ligand) superfamily, member 4
• tumor necrosis factor ligand superfamily member 4
• TXGP1

Background

CD252 is a 35 kD member of the TNF superfamily also known as OX40L and CD134L. The OX40 ligand is expressed on activated B cells and antigen presenting cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to increase proliferation and IL-2 production, and to enhance proliferation and immunoglobulin secretion in activated B cells. The RM134L antibody can inhibit OX40/OX40 ligand interactions and block the costimulatory activity of OX40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OX40 Ligand/TNFSF4 Antibody (NBP2-11969)(2)

Publications using NBP2-11969

• Kawana Y, Suga H, Kamijo H Et al. Roles of OX40 and OX40 Ligand in Mycosis Fungoides and Sezary Syndrome International journal of molecular sciences 2021-11-22 [PMID: 34830466] (Human)
• Byun M, Ma CS, Akcay A et al. Inherited Human OX40 deficiency underlying classic Kaposi sarcoma of childhood. J Exp Med 2013-07-29 [PMID: 23897980] (IHC-Fr, Human)

FAQs for OX40 Ligand/TNFSF4 Antibody (NBP2-11969). (Showing 1 - 2 of 2 FAQs).

1. Could you clarify what 'guaranteed for this application on human samples' means? Can I return the antibody if it does not work for IHC-P in our hands?
   • We stand behind our products 100% and that statement is refering to our Novus' Quality Guarantee.
2. I have bought a TNFSF4 antibody (MM0505-8S23) from you. Now I would like to be sure, that I reconstitute the lyophilized antibody the right way. Could you please tell me, with which fluid I am supposed to reconstitute it and what the final concentration should be?
   • To reconstitute our TNFSF4 antibody with catalogue number NBP2-11969 you should centrifuge the vial and then add 200ul sterile PBS. The final concentration is 500ug/ml. Reconstituted antibody can be aliquoted and stored frozen at <-20 for at least for six months without detectable loss of activity. You should avoid repeated freeze-thaw cycles.

Bioinformatics

• Gene Symbol: TNFSF4
• Uniprot:
  • P23510()