Otx1 Antibody (1F2)

Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of monoclonal antibody to OTX1 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi ( Cat # H00005013-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 (Cat # H00005013-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: Otx1 Antibody (1F2) [H00005013-M01] - Detection limit for recombinant GST tagged OTX1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 1F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Otx1 Antibody (1F2) Summary

• Immunogen: OTX1 (NP_055377, 10 a.a. ~ 116 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YGMNGLGLAGPAMDLLHPSVGYPATPRKQRRERTTFTRSQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSGTKSRPAKKKSSPVR
• Specificity: OTX1 (1F2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTX1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Otx1 Antibody (1F2)

• FLJ38361
• homeobox protein OTX1
• MGC15736
• orthodenticle (Drosophila) homolog 1
• orthodenticle homeobox 1
• orthodenticle homolog 1 (Drosophila)
• Orthodenticle homolog 1

Background

This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper brain and sensory organ development and can cause epilepsy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTX1
• Entrez:
  • 5013(Human)
• OMIM:
  • 600036(Human)
• Uniprot:
  • NP_055377(Human)