Otubain-2 Antibody

Western Blot: Otubain-2 Antibody [NBP1-20958] - (1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Otubain-2 Antibody Summary

• Description: Novus Biologicals Goat Otubain-2 Antibody (NBP1-20958) is a polyclonal antibody validated for use in WB and ELISA. Anti-Otubain-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YKTSHYNILYAADKH corresponding to internal region according to NP_075601.1.
• Epitope: C terminus
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OTUB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 27 kDa band observed in human brain (cerebellum) lysates (calculated MW of 27.2 kDa band according to NP_075601.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Otubain-2 Antibody

• C14orf137
• C14orf137chromosome 14 open reading frame 137
• Deubiquitinating enzyme OTUB2
• EC 3.4.19.12
• FLJ21916
• MGC3102
• OTB2otubain-2
• OTU domain, ubiquitin aldehyde binding 2
• OTU domain-containing ubiquitin aldehyde-binding protein 2
• OTU2
• OTU2ubiquitin thioesterase OTUB2
• OTUB2
• Otubain2
• Otubain-2
• ubiquitin-specific protease otubain 2
• Ubiquitin-specific-processing protease OTUB2

Background

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otubain-2 Antibody (NBP1-20958)(1)

Publication using NBP1-20958

• Nanao MH, Tcherniuk SO, Chroboczek J et al. Crystal structure of human otubain 2. EMBO Rep 2004-08-01 [PMID: 15258613]

Bioinformatics

• Gene Symbol: OTUB2
• Entrez:
  • 78990(Human)
  • 68149(Mouse)
  • 314405(Rat)
• Uniprot:
  • Q96DC9(Human)