Otubain-2 Antibody (2E7) - Azide and BSA Free

Sandwich ELISA: Otubain-2 Antibody (2E7) [H00078990-M14] - Detection limit for recombinant GST tagged OTUB2 is 1 ng/ml as a capture antibody.

Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14] - Analysis of OTUB2 transfected lysate using anti-OTUB2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OTUB2 rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: ELISA, IP
• Clone: 2E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Otubain-2 Antibody (2E7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Otubain-2 Antibody (2E7) - Azide and BSA Free (H00078990-M14) is a monoclonal antibody validated for use in WB, ELISA and IP. Anti-Otubain-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: OTUB2 (NP_075601, 126 a.a. ~ 234 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
• Specificity: OTUB2 - OTU domain, ubiquitin aldehyde binding 2 (2E7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OTUB2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
• Application Notes: This antibody is useful for ELISA.WB was previously validated through publication (30241937) for a previous lot. Current lot has been reported by several customers to not work in WB and is not recommended.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Otubain-2 Antibody (2E7) - Azide and BSA Free

• C14orf137
• C14orf137chromosome 14 open reading frame 137
• Deubiquitinating enzyme OTUB2
• EC 3.4.19.12
• FLJ21916
• MGC3102
• OTB2otubain-2
• OTU domain, ubiquitin aldehyde binding 2
• OTU domain-containing ubiquitin aldehyde-binding protein 2
• OTU2
• OTU2ubiquitin thioesterase OTUB2
• OTUB2
• Otubain2
• Otubain-2
• ubiquitin-specific protease otubain 2
• Ubiquitin-specific-processing protease OTUB2

Background

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otubain-2 Antibody (H00078990-M14)(1)

Publication using H00078990-M14

• Li XY, Mao XF, Tang XQ et al. Regulation of Gli2 stability by deubiquitinase OTUB2. Biochem. Biophys. Res. Commun. 2018-09-18 [PMID: 30241937] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: OTUB2
• Entrez:
  • 78990(Human)
• Uniprot:
  • NP_075601(Human)