ORC6L Antibody - BSA Free

Immunoprecipitation: ORC6L Antibody [NB100-74619] - Detection of Human ORC6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ORC6 antibody NB100-74619 used for IP at 3 mcg/mg lysate. ORC6 was also immunoprecipitated by rabbit anti-ORC6 antibody NB100-74620, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ORC6L Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ORC6L Antibody - BSA Free (NB100-74619) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 200 and 230 of human origin recognition complex subunit 6 using the numbering given in entry NP_055136.1 (GeneID 23594).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is not recommended for Western Blot.
• Control:

  ORC6L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ORC6L Antibody - BSA Free

• ORC6Lorigin recognition complex, subunit 6 homolog-like (yeast)
• origin recognition complex subunit 6
• origin recognition complex, subunit 6 like (yeast)
• origin recognition complex, subunit 6

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORC6
• Entrez:
  • 23594(Human)
• Uniprot:
  • NP_055136.1(Human)