Orai1 Antibody - BSA Free

Western Blot: Orai1 Antibody - BSA Free [NBP1-77289] - Human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289] - Analysis of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 ug/ml.

Immunohistochemistry: Orai1 Antibody - BSA Free [NBP1-77289] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: Orai1 Antibody - BSA Free [NBP1-77289] - Immunofluorescence of Orai1 in human ovary tissue with Orai1 antibody at 20 ug/mL.Red: Orai1 Antibody Blue: DAPI staining

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Orai1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Orai1 Antibody - BSA Free (NBP1-77289) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Orai1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1. The immunogen is located within the last 50 amino acids of ORAI1. Amino Acid Squence: QDQLDHRGDHPLTPG
• Specificity: This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
• Predicted Species: Rat (93%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORAI1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE.
• Control Peptide:

  Orai1 Peptide (NBP1-77289PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Orai1 Antibody - BSA Free

• CRACM1
• CRACM1ORAT1
• FLJ14466
• ORAI calcium release-activated calcium modulator 1
• Orai1
• ORAT1
• Protein orai-1
• TMEM142A
• TMEM142Acalcium release-activated calcium modulator 1
• Transmembrane protein 142Acalcium release-activated calcium channel protein 1

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Orai1 Antibody (NBP1-77289)(2)

Publications using NBP1-77289

• Angulo J, Fern�ndez A, Sevilleja-Ortiz A et al. Upregulation of Orai Channels Contributes to Aging-Related Vascular Alterations in Rat Coronary Arteries International Journal of Molecular Sciences 2023-08-29 [PMID: 37686206] (Immunohistochemistry, Human, Rat)
• Sevilleja-Ortiz A, El Assar M, GarcIa-Rojo E Et al. Ageing-induced hypercontractility is related to functional enhancement of STIM/Orai and upregulation of Orai 3 in rat and human penile tissue Mech Ageing Dev 2021-10-26 [PMID: 34699858] (IF/IHC, Human, Rat)
  
  Details:
  Citation using the HRP version of this antibody.

Bioinformatics

• Gene Symbol: ORAI1
• Entrez:
  • 84876(Human)
• Uniprot:
  • Q96D31(Human)