Oncostatin M/OSM Antibody (824157) [Unconjugated]

Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Oncostatin M/OSM (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Oncostatin M/OSM Monoclonal Antibody (Catalog # MAB4951). The ND50 is typically 0.2-1.2 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 824157
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Oncostatin M/OSM Antibody (824157) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Oncostatin M/OSM
  Ala24-Arg206
  Accession # P53347
• Specificity: Detects mouse Oncostatin M/OSM in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Osm
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-1.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oncostatin M/OSM Antibody (824157) [Unconjugated]

• MGC20461
• oncostatin M
• oncostatin-M
• OSM

Background

Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and Cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and Erythropoietin. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mouse OSM. The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha . It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.

1. Yoshimura, A. et al. (1996) EMBO J. 15:1055.
2. Ray, P. et al. (1996) Endocrinology 137:1151.
3. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oncostatin M/OSM Antibody (MAB4951)(1)

Publication using MAB4951

• M Simonneau, E Frouin, V Huguier, C Jermidi, JF Jégou, J Godet, A Barra, I Paris, P Levillain, S Cordier-Di, N Pedretti, FX Bernard, JC Lecron, F Morel, L Favot Oncostatin M is overexpressed in skin squamous-cell carcinoma and promotes tumor progression Oncotarget, 2018-11-23;9(92):36457-36473. 2018-11-23 [PMID: 30559930] (IHC, Mouse)

Review for Oncostatin M/OSM Antibody (MAB4951) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Laure FAVOT LAFORGE ICC Mouse 03/30/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Ear Skin (frozen section)
• Species: Mouse
• Lot: CJUB0217011

Comments

• Comments: Frozen slide
  Fixation: faraformaldhehyde 4%
  mouse ear skin injected with OSM adenovirus

Bioinformatics

• Gene Symbol: Osm
• Uniprot:
  • P53347()