OGG1 Antibody

Product Discontinued

OGG1 Antibody Summary

• Immunogen: Peptide with sequence C-HPKTSQAKGPSPLANK corresponding to internal region according to NP_035087.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OGG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in rat spleen lysates (calculated MW of 38.7 kDa band according to rat NP_110497.1 and 38.8 kDa band according to mouse NP_035087.2).
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OGG1 Antibody

• 8-hydroxyguanine DNA glycosylase
• 8-oxoguanine DNA glycosylase
• AP lyase
• DNA-apurinic or apyrimidinic site lyase
• HOGG1
• MMH
• MUTMOGH1HMMH
• N-glycosylase/DNA lyase
• OGG1

Background

The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at 8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6 diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. N glycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion. Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N terminal region in common. Many alternative splice variants for this gene have been described, but the full length nature for every variant has not been determined. The N terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OGG1 Antibody (NB100-61664)(1)

Publication using NB100-61664

• Kovtun IV, Liu Y, Bjoras M et al. OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells. Nature 2007-05-24 [PMID: 17450122]

Bioinformatics

• Gene Symbol: OGG1
• Entrez:
  • 18294(Mouse)
  • 81528(Rat)
• Uniprot:
  • O08760(Mouse)