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OCT2 Antibody (Oct2/2137) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-P
Clone
Oct2/2137
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75735AF350
Alexa Fluor 405
NBP2-75735AF405
Alexa Fluor 488
NBP2-75735AF488
Alexa Fluor 532
NBP2-75735AF532
Alexa Fluor 594
NBP2-75735AF594
Alexa Fluor 647
NBP2-75735AF647
Alexa Fluor 700
NBP2-75735AF700
Alexa Fluor 750
NBP2-75735AF750
Biotin
NBP2-75735B
DyLight 350
NBP2-75735UV
DyLight 405
NBP2-75735V
DyLight 488
NBP2-75735G
DyLight 550
NBP2-75735R
DyLight 594
NBP2-75735DL594
DyLight 650
NBP2-75735C
DyLight 680
NBP2-75735FR
DyLight 755
NBP2-75735IR
HRP
NBP2-75735H
Janelia Fluor 549
NBP2-75735JF549
Janelia Fluor 646
NBP2-75735JF646
Unconjugated
NBP2-75734-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-75734-100ug
Azide and BSA Free
NBP2-75735
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OCT2 Antibody (Oct2/2137) [FITC] Summary

Immunogen
Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)
Localization
Nuclear
Specificity
OCT2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. OCT2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of OCT2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of OCT2. Several studies of OCT2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for OCT2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2F2
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for OCT2 Antibody (Oct2/2137) [FITC]

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2Oct-2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF-2
  • OTF2POU domain class 2, transcription factor 2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677] - Analysis of OCT-1 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.
Species: Ca, Eq, Hu, Pm
Applications: ICC/IF, IHC, IHC-P, WB
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(2C5)
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Flow Cytometry: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis of Jurkat cells using SLC22A1 mouse mAb (green) and negative control (purple).
Western Blot: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis using SLC22A1 mAb against HEK293 (1) and SLC22A1(AA: 284-347)-hIgGFc transfected HEK293 (2) cell lysate.
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BOB1 Antibody
(BOB1/2424)
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Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PA, WB
Host Cell Factor
1/HCFC1 Antibody [Unc ...
AF6254
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Host Cell Factor 1/HCFC1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Host Cell Factor 1/HCFC1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Publication)
Species: Hu
Applications: ICC, IHC, WB
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Antibody
NBP1-89417
Immunohistochemistry-Paraffin: SLC22A2/OCT2 Antibody [NBP1-89417] - Analysis in human kidney and liver tissues. Corresponding SLC22A2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC22A2/OCT2 Antibody [NBP1-89417] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: SLC22A2/OCT2 Antibody [NBP1-89417] - Staining of human kidney shows moderate to strong cytoplasmic/membranous positivity in cells in tubules.
(2 Publications)
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Applications: IHC, IHC-P, Single-Cell Western, WB
Oct-3/4 Antibody
[Unconjugated]
AF1759
BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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NBP1-83976
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Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human fallopian tube shows moderate membranous and cytoplasmic positivity in glandular cells.
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c-jun [p Ser63]
Antibody (SY0297)
NBP2-67471
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Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the66-440 kDa separation system.
(1 Review)
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Pax5/BSAP Antibody
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Flow Cytometry: Pax5/BSAP Antibody [NBP2-29905] - Analysis of PAX5 (red histogram) on Ramos cells. Cells were harvested, fixed with 4% formaldehyde, permeabilized, washed with PBS, and incubated with a PAX5 polyclonal antibody at a 1:50 dilution or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 488 goat anti-rabbit IgG secondary antibody was performed. 30,000 cells were acquired for analysis.
Immunocytochemistry/Immunofluorescence: Pax5/BSAP Antibody [NBP2-29905] - Analysis of PAX5 (green) showing staining in the in the nucleus of Ramos cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/Immunofluorescence: Pax5/BSAP Antibody [NBP2-29905] - Analysis of PAX5 (green) showing staining in the in the nucleus of Raji cells (right) compared to a negative control without primary antibody (left).
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Immunohistochemistry-Paraffin: MATE1 Antibody [NBP1-87909] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: MATE1 Antibody [NBP1-87909] - Staining of human kidney shows strong membranous positivity in cells in tubules.
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Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: c-Fos Antibody [NBP1-89065] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.
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CD20 Antibody
(AISB12) - BSA Free
NBP1-43435
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Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.
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IL-2 [Unconjugated]
202-IL/CF
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
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Cytokeratin 20
Antibody (SA35-03)
NBP2-67309
Flow Cytometry: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of CRC cells with Cytokeratin 20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
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Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
SLC22A6 Antibody
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Immunohistochemistry-Paraffin: SLC22A6 Antibody [NBP2-62690] - Analysis in human kidney and lymph node tissues using Anti-SLC22A6 antibody. Corresponding SLC22A6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SLC22A6 Antibody [NBP2-62690] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: SLC22A6 Antibody [NBP2-62690] - Staining of human kidney shows high expression.
Species: Hu
Applications: IHC, IHC-P
NFkB p50 (NLS)
Inhibitor Peptide Set
NBP2-29323
Functional (Inhibition): NFkB p105/p50 Inhibitor Peptide Set [NBP2-29323] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29323) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).
(12 Publications)
Species: Bv, Ca, Ch, Hu, Mu, Rt, Xp
Applications: Func-Inh

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Bioinformatics

Gene Symbol POU2F2