Reactivity | HuSpecies Glossary |
Applications | WB, ELISA, IHC, IHC-P, PA |
Clone | Oct2/2137 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1 mg/ml |
Immunogen | Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary) |
Localization | Nuclear |
Specificity | OCT2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. OCT2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of OCT2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of OCT2. Several studies of OCT2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for OCT2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | POU2F2 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | Positive Control: Raji or U-937 cells. Spleen or Tonsil. ELISA: For coating, order BSA Free version of this antibody. Immunohistochemistry-Paraffin: 1-2 ug/mL for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-75735AF350 | |
Alexa Fluor 405 | NBP2-75735AF405 | |
Alexa Fluor 488 | NBP2-75735AF488 | |
Alexa Fluor 532 | NBP2-75735AF532 | |
Alexa Fluor 594 | NBP2-75735AF594 | |
Alexa Fluor 647 | NBP2-75735AF647 | |
Alexa Fluor 700 | NBP2-75735AF700 | |
Alexa Fluor 750 | NBP2-75735AF750 | |
Biotin | NBP2-75735B | |
DyLight 350 | NBP2-75735UV | |
DyLight 405 | NBP2-75735V | |
DyLight 488 | NBP2-75735G | |
DyLight 550 | NBP2-75735R | |
DyLight 594 | NBP2-75735DL594 | |
DyLight 650 | NBP2-75735C | |
DyLight 680 | NBP2-75735FR | |
DyLight 755 | NBP2-75735IR | |
FITC | NBP2-75735F | |
HRP | NBP2-75735H | |
Janelia Fluor 549 | NBP2-75735JF549 | |
Janelia Fluor 646 | NBP2-75735JF646 |
Diseases for OCT2 Antibody (NBP2-75735)Discover more about diseases related to OCT2 Antibody (NBP2-75735).
| Pathways for OCT2 Antibody (NBP2-75735)View related products by pathway.
|
PTMs for OCT2 Antibody (NBP2-75735)Learn more about PTMs related to OCT2 Antibody (NBP2-75735).
| Research Areas for OCT2 Antibody (NBP2-75735)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.