OCT2 Antibody (OCT2/2137) - Azide and BSA Free

Western Blot: OCT2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Western Blot Analysis (A) Recombinant Protein (B) Daudi cell lysate Oct-2 Mouse Monoclonal Antibody (OCT2/2137).

Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).

Western Blot: OCT2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Western Blot Analysis Ramos cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).

Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).

Protein Array: OCT2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human lymph node stained with OCT2 antibody (OCT2/2137). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, MA
• Clone: OCT2/2137
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

OCT2 Antibody (OCT2/2137) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75734).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Immunogen: Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)
• Localization: Nuclear
• Marker: B-Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POU2F2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 - 2 ug/mL
  • Immunohistochemistry 1 - 2 ug/mL
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
  • Protein Array
  • Western Blot 2 ug/mL
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for OCT2 Antibody (OCT2/2137) - Azide and BSA Free

• homeobox protein
• Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
• OCT2Oct-2
• Octamer-binding protein 2
• Octamer-binding transcription factor 2
• OTF-2
• OTF2POU domain class 2, transcription factor 2
• POU class 2 homeobox 2
• POU domain, class 2, transcription factor 2

Background

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU2F2
• Uniprot:
  • P09086(Human)