BOB1 Antibody (BOB1/2424)

Images

 
Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells using BOB1 Antibody (BOB1/2424).followed by goat anti-Mouse IgG ...read more
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Protein Array: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Protein Array containing >19,000 full-length human proteins using BOB1 Antibody (BOB1/2424) Z- and S- Score: The Z-score represents the strength of a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PA
Clone
BOB1/2424
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

BOB1 Antibody (BOB1/2424) Summary

Immunogen
Recombinant human BOB1 (POU2AF1) protein fragment (around aa 148-255) (exact sequence is proprietary)
Localization
Nuclear
Specificity
BOB1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2AF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Positive Control: Ramos cells. Spleen or Tonsil Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for BOB1 Antibody (BOB1/2424)

  • B-cell-specific coactivator OBF-1
  • BOB-1
  • OBF-1
  • OBF1BOB1
  • OCAB
  • OCA-B
  • OCT-binding factor 1
  • POU class 2 associating factor 1
  • POU domain class 2, associating factor 1
  • POU domain class 2-associating factor 1
  • POU domain, class 2, associating factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BOB1 Antibody (NBP2-75752) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75753AF350
Alexa Fluor 405 NBP2-75753AF405
Alexa Fluor 488 NBP2-75753AF488
Alexa Fluor 532 NBP2-75753AF532
Alexa Fluor 594 NBP2-75753AF594
Alexa Fluor 647 NBP2-75753AF647
Alexa Fluor 700 NBP2-75753AF700
Alexa Fluor 750 NBP2-75753AF750
Biotin NBP2-75753B
DyLight 350 NBP2-75753UV
DyLight 405 NBP2-75753V
DyLight 488 NBP2-75753G
DyLight 550 NBP2-75753R
DyLight 594 NBP2-75753DL594
DyLight 650 NBP2-75753C
DyLight 680 NBP2-75753FR
DyLight 755 NBP2-75753IR
FITC NBP2-75753F
HRP NBP2-75753H
Janelia Fluor 549 NBP2-75753JF549
Janelia Fluor 646 NBP2-75753JF646

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Winter Protein Games
Get into the Winter Protein Games 2014! Check out the contenders competing including SKI, POLE, ICEBURG, SKT, TRAIL, WIN and BOB1. Learn more about each protein's function, gene name, molecular weight and family.View all the fun and games happening...  Read full blog post.

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Bioinformatics

Gene Symbol POU2AF1
Uniprot