Occludin Antibody (CL1567) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line CACO-2Lane 3: Human cell line U-87

Staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Staining of CACO-2 cells using the Anti-Occludin monoclonal antibody) .

Staining of human fallopian tube shows strong membranous immunoreactivity in glandular cells.

Staining of human liver shows membranous immunoreactivity in hepatocytes.

Staining of human kidney shows membranous positivity in renal tubules.

Staining of human prostate cancer shows membranous positivity in tumor cells.

Staining of human tonsil shows membranous immunoreactivity in squamous epithelium cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL1567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Occludin Antibody (CL1567) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q16625, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: OCLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Occludin Antibody (CL1567) - Azide and BSA Free

• BLCPMG
• Occludin
• OCLN

Background

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCLN