Occludin Antibody (CL1567) - BSA Free

Western Blot: Occludin Antibody (CL1567) [NBP2-52972] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1567) [NBP2-52972] - Staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human tonsil shows membranous immunoreactivity in squamous epithelium cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human fallopian tube shows strong membranous immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human kidney shows membranous positivity in renal tubules.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human liver shows membranous immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1567) [NBP2-52972] - Staining of human prostate cancer shows membranous positivity in tumor cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL1567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Occludin Antibody (CL1567) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: OCLN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  Occludin Recombinant Protein Antigen (NBP2-52972PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Occludin Antibody (CL1567) - BSA Free

• BLCPMG
• Occludin
• OCLN

Background

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OCLN