OAT Antibody (23A2) - BSA Free

Western Blot: OAT Antibody (23A2) [NBP1-49312] - PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: OAT Antibody (23A2) [NBP1-49312] - Human HeLa cells stained with monoclonal anti-human OAT antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 23A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OAT Antibody (23A2) - BSA Free Summary

• Description: Novus Biologicals Mouse OAT Antibody (23A2) - BSA Free (NBP1-49312) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human OAT (33-439aa) purified from E. coli.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OAT
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Western Blot 1:250-1:500

Reactivity Notes

May react with Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for OAT Antibody (23A2) - BSA Free

• GACR
• HOGA
• OAT ornithine aminotransferase
• OATASE
• OKT

Background

OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OAT
• Entrez:
  • 4942(Human)
• Uniprot:
  • NP_000265(Human)