OAT Antibody (23A2)

Images

 
Western Blot: OAT Antibody (AT23A2) [NBP1-49312] - PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ...read more
Immunocytochemistry/ Immunofluorescence: OAT Antibody (AT23A2) [NBP1-49312] - Human HeLa cells stained with monoclonal anti-human OAT anitbody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
23A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

OAT Antibody (23A2) Summary

Immunogen
Recombinant human OAT (33-439aa) purified from E. coli.
Specificity
Human OAT (33-439aa).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OAT
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:250
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250-500.

Reactivity Notes

Human. May react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for OAT Antibody (23A2)

  • GACR
  • HOGA
  • OAT ornithine aminotransferase
  • OATASE
  • OKT

Background

OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OAT Antibody (NBP1-49312) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49312AF405
Alexa Fluor 488 Labeled NBP1-49312AF488
Alexa Fluor 647 Labeled NBP1-49312AF647
Alexa Fluor 700 Labeled NBP1-49312AF700
DyLight 350 Labeled NBP1-49312UV
DyLight 405 Labeled NBP1-49312V
DyLight 488 Labeled NBP1-49312G
DyLight 680 Labeled NBP1-49312FR
DyLight 755 Labeled NBP1-49312IR

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Bioinformatics

Gene Symbol OAT
Entrez
Uniprot