OASIS/CREB3L1 Antibody

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human OASIS/CREB3L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for OASIS/CREB3L1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CREB3L1 is required for metastasis and is upregulated in the metastases of breast cancer patients. a A quantification of circulating tumor cells (CTCs) per ml of blood from NOD/SCID mice 7 weeks after being transplanted with MDA.MB.231-Luc-LM2 cells transduced with shRNAs targeting LacZ or CREB3L1, n = 5. b Lung metastases of the animals in a were quantified (top) by radiance of the whole lung in each animal, n = 5. Total radiance was also imaged (bottom). c Lung metastases of the animals in a was gauged by immunohistochemistry staining for GFP-positive cancer cells in lung sections. GFP area per field was quantified (top) and imaged (bottom), n = 10,10,9 for Ctrl, sh1, and sh2, respectively (scale bar: 40 µm). d Expression of CREB3L1 in 529 primary breast cancer tissues and 45 breast cancer metastases (GSE20565, GSE20685, GSE7904, and GSE3744) shown as a box-and-whiskers plot. e Immunohistochemical staining for CREB3L1 in a tissue microarray containing primary breast cancer tissues (left, n = 50) and breast cancer metastases in lymph nodes (middle, n = 50). Nuclear CREB3L1 levels were quantified (right). Scale bar: 40 µm. Data are represented as mean ± SEM or the mean alone. * indicates p < 0.05; ** indicates p < 0.01 (Student’s t-test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29057869), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Discontinued

OASIS/CREB3L1 Antibody Summary

• Immunogen: E. coli-derived recombinant human OASIS/CREB3L1
  Met1-Asp111
  Accession # Q96BA8
• Specificity: Detects human OASIS/CREB3L1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CREB3L1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OASIS/CREB3L1 Antibody

• BBF-2 Homolog
• cAMP responsive element binding protein 3-like 1
• cAMP-responsive element-binding protein 3-like protein 1
• CREB3L1
• OASIS
• OASIScyclic AMP-responsive element-binding protein 3-like protein 1
• old astrocyte specifically induced substance
• Old astrocyte specifically-induced substance

Background

OASIS (old astrocyte specifically-induced substance) is a 57‑60 kDa member of the CREB/ATF family of transcription factors. Human OASIS is 519 amino acids (aa) in length. It is an ER transmembrane protein with a cytosolic N-terminus (aa 1‑374) and a luminal C‑terminus. The N-terminus contains one b-Zip region (aa 292‑353). Misfolded proteins in the ER induce proteolytic cleavage of OASIS. The released 66 kDa N-terminus translocates to the nucleus to activate stress response genes. There is one alternate splice form that shows a deletion of aa 112‑198. Over aa 1‑111, human OASIS shares 96% aa sequence identity with mouse OASIS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OASIS/CREB3L1 Antibody (AF4080)(1)

Publication using AF4080

• Greenwood M, Greenwood M, Paton J, Murphy D Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. PLoS ONE, 2015-04-27;10(4):e0124956. 2015-04-27 [PMID: 25915053] (IHC, Rat)

Bioinformatics

• Gene Symbol: CREB3L1
• Uniprot:
  • Q96BA8()