O-GlcNAcase/OGA/MGEA5 293T Cell Transient Overexpression Lysate

MGEA5 transfected lysate.

Lane 1: MGEA5 transfected lysate (100.87 KDa) Lane 2: Non-transfected lysate.

Product Discontinued

O-GlcNAcase/OGA/MGEA5 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-MGEA5 antibody by Western Blots. Plasmid: pCMV-MGEA5 full-length
• Specificity: O-GlcNAcase/OGA/MGEA5 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: OGA

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for O-GlcNAcase/OGA/MGEA5 293T Cell Transient Overexpression Lysate

• bifunctional protein NCOAT
• FLJ11229
• GH84
• HEXC
• HEXC3
• Hexosaminidase B
• hyaluronidase in meningioma
• KIAA0679
• MEA5FLJ23355
• meningioma expressed antigen 5 (hyaluronidase)
• Meningioma-expressed antigen 5
• MGEA5
• NCOAT
• Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• OGA
• OGlcNAcase
• O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: OGA