NUP62 Antibody (RL31)

Western Blot: NUP62 Antibody (RL31) [NB120-2736] - Analysis of Nucleoporin p62 was performed by loading whole cell lysates (20ug) of the indicated samples and 5ul of prestained protein ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody at a dilution of 1:200 overnight at 4C on a rocking platform, followed by a goat anti-Armenian hamster IgG-HRP secondary antibody at a dilution of 1:10,000 for at least 30 minutes at room temperature.

• Reactivity: Rt
• Applications: WB, ICC/IF
• Clone: RL31
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NUP62 Antibody (RL31) Summary

• Description: Novus Biologicals Armenian Hamster NUP62 Antibody (RL31) (NB120-2736) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.
• Localization: Central region of the nuclear pore. During mitotic cell division, it associates with the poles of the mitotic spindle.
• Specificity: Detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat tissues.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: NUP62
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:50
  • Western Blot 1:200
• Application Notes: WB: Detects a single 62 kDa protein representing nucleoporin p62 in rat liver nuclear envelope fractions. IF: Staining of p62 in rat liver with this antibody results in staining of both the nucleoplasmic and cytoplasmic surfaces of the nuclear pore complex (NPC).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2-7.4) with 1mg/ml BSA and 30% glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for NUP62 Antibody (RL31)

• DKFZp547L134,62 kDa nucleoporin
• FLJ20822
• FLJ43869
• IBSNp62
• MGC841
• nuclear pore glycoprotein p62
• nucleoporin 62kD
• nucleoporin 62kDa
• SNDINucleoporin Nup62

Background

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUP62
• Entrez:
  • 65274(Rat)
• Uniprot:
  • P17955(Rat)