NUP62 Antibody (252) - Azide and BSA Free

Western Blot: Nucleoporin p62 Antibody (252) [NBP2-43589] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Nucleoporin p62 antibody [252] dilution: 1:1000

Nucleoporin p62 antibody [GT252] detects Nucleoporin p62 protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody [GT252] (NBP2-43589) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF
• Clone: 252
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NUP62 Antibody (252) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NUP62 Antibody (252) - Azide and BSA Free (NBP2-43589) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NUP62. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUP62
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (85%), Rat (84%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for NUP62 Antibody (252) - Azide and BSA Free

• DKFZp547L134,62 kDa nucleoporin
• FLJ20822
• FLJ43869
• IBSNp62
• MGC841
• nuclear pore glycoprotein p62
• nucleoporin 62kD
• nucleoporin 62kDa
• SNDINucleoporin Nup62

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUP62