NUP50 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.

Western Blot: NUP50 Antibody [NBP2-19610] - 50 ug mouse testis lysate/extract 10% SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000.

Western Blot: NUP50 Antibody [NBP2-19610] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (NBP2-19610) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Bv, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NUP50 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NUP50 Antibody - BSA Free (NBP2-19610) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.
• Localization: Nucleus; nuclear pore complex; Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity)
• Predicted Species: Bovine (100%), Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP50
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NUP50 Antibody - BSA Free

• 50 kDa nucleoporin
• MGC39961
• NPAP60
• NPAP60LNucleoporin Nup50
• nuclear pore complex protein Nup50
• Nuclear pore-associated protein 60 kDa-like
• nuclear pore-associated protein 60L
• nucleoporin 50kD
• nucleoporin 50kDa

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NUP50 Antibody (NBP2-19610)(1)

Publication using NBP2-19610

• Rossi A, Coum A, Madelenat M, et al. Neural stem cells alter nucleocytoplasmic partitioning and accumulate nuclear polyadenylated transcripts during quiescence bioRxiv 2021-01-07 (WB)

Bioinformatics

• Gene Symbol: NUP50
• Uniprot:
  • Q9UKX7(Human)