NUP50 Antibodies
We offer NUP50 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each NUP50 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NUP50 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the NUP50 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NUP50 polyclonal antibodies and browse our NUP50 monoclonal antibody catalog.
Alternate Names for NUP50 Antibodies
anti-NUP50 antibody, anti-50 kDa nucleoporin antibody, anti-MGC39961 antibody, anti-NPAP60 antibody, anti-NPAP60LNucleoporin Nup50 antibody, anti-nuclear pore complex protein Nup50 antibody, anti-Nuclear pore-associated protein 60 kDa-like antibody, anti-nuclear pore-associated protein 60L antibody, anti-nucleoporin 50kD antibody, anti-nucleoporin 50kDa antibody
10 Results for "nup50" in Primary Antibodies
![Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-93324-img0006.jpg "Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NUP50 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-NB100-93324-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NB100-93324] - Detection of Human NUP50 on HeLa whole cell lysate using NB100-93324. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody NB100-93323.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-93324-img0002.jpg)
![Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NB100-1212-img0005.jpg "Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NB100-1212-img0004.jpg)
![Western Blot: NUP50 Antibody [NBP2-13683] - Analysis using Anti-NUP50 antibody NBP2-13683 (A) shows similar pattern to independent antibody NBP2-57627 (B).](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-13683-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NBP2-13683] - Analysis using Anti-NUP50 antibody NBP2-13683 (A) shows similar pattern to independent antibody NBP2-57627 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-13683] - Staining of human cell line MCF7 shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13683-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining in human testis and heart muscle tissues using anti-NUP50 antibody. Corresponding NUP50 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0008.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining of human heart muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0006.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-13683] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-13683-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19610-img0004.jpg "Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-19610-img0003.jpg)
![Western Blot: NUP50 Antibody [NBP2-19610] - 50 ug mouse testis lysate/extract 10% SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000.](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-19610-img0001.jpg)
![Western Blot: NUP50 Antibody [NBP2-19610] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (NBP2-19610) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19610_rabbit-polyclonal-nup50-antibody-910202420122012.jpg)
![Western Blot: NUP50 Antibody (SR1992) [NBP3-22562] - Western blot analysis using NBP3-22562 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22562_rabbit-nup50-mab-sr1992-57202315584831.jpg "Western Blot: NUP50 Antibody (SR1992) [NBP3-22562] - Western blot analysis using NBP3-22562 in HeLa cell lysate.")
![Western Blot: NUP50 Antibody (JE64-06) [NBP3-32671] - Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32671) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32671_rabbit-nup50-mab-je64-06-278202416285296.jpg "Western Blot: NUP50 Antibody (JE64-06) [NBP3-32671] - Western blot analysis of NUP50 on different lysates with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32671) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUP50 Antibody (JE64-06) [NBP3-32671] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-NUP50 antibody (NBP3-32671) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32671) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32671_rabbit-nup50-mab-je64-06-29820241435229.jpg)
![Western Blot: NUP50 Antibody [NBP2-57627] - Analysis using Anti-NUP50 antibody NBP2-57627 (A) shows similar pattern to independent antibody NBP2-13683 (B).](http://images.novusbio.com/fullsize/NUP50-Antibody-Western-Blot-NBP2-57627-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NUP50 Antibody [NBP2-57627] - Analysis using Anti-NUP50 antibody NBP2-57627 (A) shows similar pattern to independent antibody NBP2-13683 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-57627] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57627-img0001.jpg)
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-97442-img0003.jpg "Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-97442] - Immunochemical staining of human NUP50 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NUP50-Antibody-Immunohistochemistry-Paraffin-NBP2-97442-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-97442] - Immunofluorescence staining of NUP50 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97442 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97442_rabbit-polyclonal-nup50-antibody-235202511254417.png)
![Western Blot: NUP50 Antibody (SR1991) [NBP3-22560] - Western blot analysis using NBP3-22560 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22560_rabbit-nup50-mab-sr1991-57202315545141.jpg "Western Blot: NUP50 Antibody (SR1991) [NBP3-22560] - Western blot analysis using NBP3-22560 in HeLa cell lysate.")
![Western Blot: NUP50 Antibody (4H7) [H00010762-M01] - NUP50 monoclonal antibody (M01), clone 4H7. Analysis of NUP50 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NUP50-Antibody-4H7-Western-Blot-H00010762-M01-img0002.jpg "Western Blot: NUP50 Antibody (4H7) [H00010762-M01] - NUP50 monoclonal antibody (M01), clone 4H7. Analysis of NUP50 expression in Hela S3 NE.")
![Sandwich ELISA: NUP50 Antibody (4H7) [H00010762-M01] - Detection limit for recombinant GST tagged NUP50 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NUP50-Antibody-4H7-Sandwich-ELISA-H00010762-M01-img0001.jpg)