NUP50 Antibody

Western Blot: NUP50 Antibody [NB100-93324] - MDA-MB-231 nuclear extract. Cells infected with lentivirus coding scramble shRNA or one of two NUP50 targeting shRNAs. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-93324] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NUP50 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: NUP50 Antibody [NB100-93324] - Detection of Human NUP50 on HeLa whole cell lysate using NB100-93324. NUP50 was also immunoprecipitated by rabbit anti-NUP50 antibody NB100-93323.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NUP50 Antibody Summary

• Description: Novus Biologicals Rabbit NUP50 Antibody (NB100-93324) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-NUP50 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 418 and 468 of human nucleoporin 50kDa using the numbering given in entry NP_009103.2 (GeneID 10762).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP50
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. NUP50 antibody validated for WB from a verified customer review.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NUP50 Antibody

• 50 kDa nucleoporin
• MGC39961
• NPAP60
• NPAP60LNucleoporin Nup50
• nuclear pore complex protein Nup50
• Nuclear pore-associated protein 60 kDa-like
• nuclear pore-associated protein 60L
• nucleoporin 50kD
• nucleoporin 50kDa

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUP50 Antibody (NB100-93324)(1)

Publication using NB100-93324

• Muller D, Telieps T, Eugster A et al. Novel minor HLA DR associated antigens in type 1 diabetes. Clin. Immunol. 2018-07-07 [PMID: 29990590] (IP, Human)

Review for NUP50 Antibody (NB100-93324) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/08/2018

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231 nuclear extract
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: NUP50
• Entrez:
  • 10762(Human)
• Uniprot:
  • Q9UKX7(Human)