![Immunocytochemistry/Immunofluorescence: SEC13 Antibody [NBP1-33291] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SEC13-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33291-img0009.jpg "Immunocytochemistry/Immunofluorescence: SEC13 Antibody [NBP1-33291] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SEC13 Antibody - BSA Free (NBP1-33291) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SEC13. The exact sequence is proprietary. |
| Predicted Species | Mouse (97%), Rat (97%), Chicken (94%), Primate (99%), Rhesus Macaque (98%), Bovine (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEC13 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SEC13 |
![Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SEC13-Antibody-Immunohistochemistry-Paraffin-NBP1-33291-img0005.jpg "Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.")
![Immunoprecipitation: SEC13 Antibody [NBP1-33291] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 ug of SEC13L1 antibody 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/SEC13-Antibody-Immunoprecipitation-NBP1-33291-img0008.jpg "Immunoprecipitation: SEC13 Antibody [NBP1-33291] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 ug of SEC13L1 antibody 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Western Blot: SEC13 Antibody [NBP1-33291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13 antibody [N1C3] (NBP1-33291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33291_rabbit-polyclonal-sec13-antibody-9102024204122.jpg "Western Blot: SEC13 Antibody [NBP1-33291] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13 antibody [N1C3] (NBP1-33291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")