Numb Overexpression Lysate (Native)

Images

 
Western Blot: NUMB Overexpression Lysate (Adult Normal) [NBL1-13870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUMB.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Numb Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216230 and based on accession number NM_003744. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens numb homolog (Drosophila) (NUMB), transcript variant 3, mRNA.
Gene
NUMB

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
69.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Numb

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Numb Overexpression Lysate (Native)

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34104
Species: Hu
Applications: IHC, IHC-P
NB100-78486
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP2-58844
Species: Hu
Applications: ICC/IF
NBP1-88861
Species: Hu, Mu
Applications: Simple Western, ICC/IF, IHC, IHC-P
NBP1-90980
Species: Hu
Applications: IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59068
Species: Hu
Applications: WB
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP2-19997
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00005725-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
NBP1-53125
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00004830-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
PP-H4417-00
Species: Hu
Applications: WB, IP, DirELISA
NLS261
Species: Hu
Applications: WB (-), IHC-P, ICC
AF755
Species: Mu
Applications: WB, Block
NBL1-13870
Species: Hu
Applications: WB

Publications for Numb Lysate (NBL1-13870) (0)

There are no publications for Numb Lysate (NBL1-13870).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Numb Lysate (NBL1-13870) (0)

There are no reviews for Numb Lysate (NBL1-13870). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Numb Lysate (NBL1-13870) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Numb Products

Bioinformatics Tool for Numb Lysate (NBL1-13870)

Discover related pathways, diseases and genes to Numb Lysate (NBL1-13870). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Numb Lysate (NBL1-13870)

Discover more about diseases related to Numb Lysate (NBL1-13870).
 

Pathways for Numb Lysate (NBL1-13870)

View related products by pathway.

PTMs for Numb Lysate (NBL1-13870)

Learn more about PTMs related to Numb Lysate (NBL1-13870).
 

Research Areas for Numb Lysate (NBL1-13870)

Find related products by research area.

Blogs on Numb

There are no specific blogs for Numb, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Numb Antibody
NB500-178

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Numb Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol NUMB
Entrez