Nucleostemin Antibody [Janelia Fluor® 646]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Janelia Fluor 646

Nucleostemin Antibody [Janelia Fluor® 646] Summary

• Immunogen: E. coli-derived recombinant rat Nucleostemin
  Lys2-Ile538
  Accession # Q811S9
• Specificity: Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by first passing the sera over a rat Nucleostemin aa 2-538 column and then passing the bound fraction over a rat Nucleostemin aa 281-538 column to removed rat Nucleostemin C-terminal specific IgG.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GNL3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Nucleostemin Antibody [Janelia Fluor® 646]

• C77032
• E2IG3NNP47
• estradiol-induced nucleotide binding protein
• GNL3
• guanine nucleotide binding protein-like 3 (nucleolar)
• guanine nucleotide-binding protein-like 3
• MGC800
• Novel nucleolar protein 47
• NSE2-induced gene 3 protein
• Nucleolar GTP-binding protein 3
• Nucleostemin

Background

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNL3