Nucleolin Antibody (NCL/7014R)

Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Human tonsil stained with Nucleolin Antibody. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Analysis of human cells administered in pig brain using Nucleolin antibody. Antibody did NOT stain porcine tissue allowing the presence of human cells to be determined. Primary antibody dilution 1:100. Image from verified customer review.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with Nucleolin antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu, Bv(-), Mu(-), Rt(-)
• Applications: WB, Flow, ICC/IF, IHC
• Clone: NCL/7014R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Nucleolin Antibody (NCL/7014R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11038)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human Nucleolin protein (Uniprot: P19338)
• Localization: Nucleolus
• Marker: Marker of Human Cells
• Specificity: Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes Optimal dilution for a specific application should be determined.
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse, Feline, Bovine, Canine or Equine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Nucleolin Antibody (NCL/7014R)

• C23
• FLJ45706
• FLJ59041
• nucleolin
• Protein C23

Background

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Nucleolin Antibody (NBP3-11037) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Pig.

reviewed by: Verified Customer IHC-P Pig 02/01/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: brain and spinal cord
• Species: Pig
• Lot: 4691-4P210225

Comments

• Comments: We were looking for human cells that were administered in pig brain. We needed an antibody that did not stain porcine tissue. This antibody did not stain pig and helped us determine the presence of human cells. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Verified Customer IHC-P Human 06/03/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human tonsil
• Species: Human
• Lot: 4691-4P210225

Comments

• Comments: Citrate pH6 retrieval at 110C, antibody dilution 1:100/30 min RT, anti-rabbit polymer detection

Bioinformatics

• Gene Symbol: NCL
• Uniprot:
  • P19338(Human)