Nucleolin Antibody (364-5)

Images

 
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). ...read more
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).
Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).
Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).

Product Details

Summary
Reactivity Hu, Bv(-), Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
364-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Nucleolin Antibody (364-5) Summary

Immunogen
lysate of SU-DHL-1 Nuclei (Uniprot: P19338)
Localization
Nucleoli
Marker
Marker of Human Cells
Specificity
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-44612 in the following applications:

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47861)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Nucleolin Antibody (364-5)

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Background

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-61646
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-85563
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NB300-269
Species: Bv, Ce, Ch, Dr, Eq, Hu, Mu, Pl, Po, Rt, Y, Ye
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, KD, WB
NBP2-48820
Species: Hu
Applications: IHC, IHC-P
NBP2-43782
Species: Hu
Applications: WB
NBP2-92342
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-59415
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-92707
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-85729
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF1029
Species: Mu
Applications: ICC, IHC, IP, WB
NBP1-82545
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
210-TA
Species: Hu
Applications: BA
NBP1-58200
Species: Hu, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-33466
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-44612
Species: Hu, Bv(-), Mu(-), Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for Nucleolin Antibody (NBP2-44612)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP2-44612 Applications Species
Tiek DM, Razaghi R, Jin L, Sadowski N Targeting destabilized DNA G-quadruplexes and aberrant splicing in drug-resistant glioblastoma bioRxiv Jul 11 1905 (ICC/IF, Human) ICC/IF Human
Tiek DM, Erdogdu B, Razaghi R et al. Temozolomide-induced guanine mutations create exploitable vulnerabilities of guanine-rich DNA and RNA regions in drug-resistant gliomas Science advances Jun 24 2022 [PMID: 35731869] (ICC/IF, Human) ICC/IF Human

Reviews for Nucleolin Antibody (NBP2-44612) (0)

There are no reviews for Nucleolin Antibody (NBP2-44612). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nucleolin Antibody (NBP2-44612) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Nucleolin Products

Bioinformatics Tool for Nucleolin Antibody (NBP2-44612)

Discover related pathways, diseases and genes to Nucleolin Antibody (NBP2-44612). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nucleolin Antibody (NBP2-44612)

Discover more about diseases related to Nucleolin Antibody (NBP2-44612).
 

Pathways for Nucleolin Antibody (NBP2-44612)

View related products by pathway.

PTMs for Nucleolin Antibody (NBP2-44612)

Learn more about PTMs related to Nucleolin Antibody (NBP2-44612).
 

Research Areas for Nucleolin Antibody (NBP2-44612)

Find related products by research area.

Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nucleolin Antibody (364-5) and receive a gift card or discount.

Bioinformatics

Gene Symbol NCL
Uniprot