Nucleolin Antibody (364-5)

Images

 
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). ...read more
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).
Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).
Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).
Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).

Product Details

Summary
Reactivity Hu, Bv(-), Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
364-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Nucleolin Antibody (364-5) Summary

Immunogen
lysate of SU-DHL-1 Nuclei
Localization
Nucleoli
Marker
Marker of Human Cells
Specificity
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-44612 in the following applications:

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-47861).

Alternate Names for Nucleolin Antibody (364-5)

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for Nucleolin Antibody (NBP2-44612)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Human
(1)
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Showing Publication 1 - 1 of 1.
Publication using NBP2-44612 Applications Species
Tiek DM, Razaghi R, Jin L, Sadowski N Targeting destabilized DNA G-quadruplexes and aberrant splicing in drug-resistant glioblastoma bioRxiv Jul 11 1905 (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nucleolin Antibody (NBP2-44612) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47861AF350
Alexa Fluor 405 NBP2-47861AF405
Alexa Fluor 488 NBP2-47861AF488
Alexa Fluor 532 NBP2-47861AF532
Alexa Fluor 594 NBP2-47861AF594
Alexa Fluor 647 NBP2-47861AF647
Alexa Fluor 700 NBP2-47861AF700
Alexa Fluor 750 NBP2-47861AF750
Allophycocyanin NBP2-47861APC
Biotin NBP2-47861B
DyLight 350 NBP2-47861UV
DyLight 405 NBP2-47861V
DyLight 488 NBP2-47861G
DyLight 550 NBP2-47861R
DyLight 594 NBP2-47861DL594
DyLight 650 NBP2-47861C
DyLight 680 NBP2-47861FR
DyLight 755 NBP2-47861IR
FITC NBP2-47861F
HRP NBP2-47861H
Janelia Fluor 549 NBP2-47861JF549
Janelia Fluor 646 NBP2-47861JF646
PE NBP2-47861PE
PerCP NBP2-47861PCP

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Bioinformatics Tool for Nucleolin Antibody (NBP2-44612)

Discover related pathways, diseases and genes to Nucleolin Antibody (NBP2-44612). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

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Bioinformatics

Gene Symbol NCL
Uniprot