NTS2/NTSR2 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

NTS2/NTSR2 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: A portion of amino acids 150-200 of human Neurotensin receptor 2 was used as the immunogen.
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTSR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted Reactivity: Rat, Dog, Bovine and Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NTS2/NTSR2 Antibody [CoraFluor™ 1]

• neurotensin receptor 2
• neurotensin receptor type 2
• neurotensin receptor, type 2
• NT-R-2
• NTR2Levocabastine-sensitive neurotensin receptor
• NTS2
• NTSR2

Background

Official Gene Symbol: NTSR2 Gen Bank Accession Number: NP_036476 Gene ID: 23620 (Human) Gene Map Locus: 2p25.1 (Human) NTR2 is a member of GPCR family. It contains a short N-terminal sequence, 7 transmembrane domains and a short cytoplasmic tail. It is a low affinity levocabastine-sensitive receptor for Neurotensin. Activation of NT2R by non-peptide agonists suggests that the receptor can couple to phospholipase C, phospholipase A2 and MAPK. Unlike NTR1, NTR2 is insensitive to GTP and has low sensitivity of sodium ions. Northern Blot analysis detected its expression in adult brain regions including the olfactory system, cerebral and crebellar cortices, hippocampus and hypothalamic nuclei. Its expression has also been found in low levels in kidney, uterus, heart and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTSR2