NTAL Antibody (NAP-07) - BSA Free

Immunocytochemistry/Immunofluorescence: NTAL Antibody (NAP-07) [NB500-341] - Subcellular localization of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. THP-1 cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
• Clone: NAP-07
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NTAL Antibody (NAP-07) - BSA Free Summary

• Description: Novus Biologicals Mouse NTAL Antibody (NAP-07) - BSA Free (NB500-341) is a monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant cytoplasmic domain (amino acids 91-244) of Human NTAL.
• Specificity: The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular) 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 2 ug/ml
• Application Notes: Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. This antibody is CyTOF ready.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NTAL Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for NTAL Antibody (NAP-07) - BSA Free

• LAB
• LABWilliams-Beuren syndrome chromosomal region 15 protein
• Linker for activation of B-cells
• linker for activation of T cells family, member 2
• linker for activation of T-cells family member 2
• Membrane-associated adapter molecule
• Non-T-cell activation linker
• NTAL
• NTALWilliams-Beuren syndrome chromosomal region 5 protein
• WBS15
• WBSCR15
• WBSCR5
• WBSCR5HSPC046
• Williams-Beuren syndrome chromosome region 5
• WSCR5

Background

The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAT2
• Entrez:
  • 7462(Human)
  • 56743(Mouse)