NTAL Antibody (440005)

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NTAL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 440005
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NTAL Antibody (440005) Summary

• Immunogen: E. coli-derived recombinant human NTAL
  Met1-Ala243
  Accession # Q9GZY6
• Specificity: Detects human NTAL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NTAL Antibody (440005)

• LAB
• LABWilliams-Beuren syndrome chromosomal region 15 protein
• Linker for activation of B-cells
• linker for activation of T cells family, member 2
• linker for activation of T-cells family member 2
• Membrane-associated adapter molecule
• Non-T-cell activation linker
• NTAL
• NTALWilliams-Beuren syndrome chromosomal region 5 protein
• WBS15
• WBSCR15
• WBSCR5
• WBSCR5HSPC046
• Williams-Beuren syndrome chromosome region 5
• WSCR5

Background

Non-T cell activation linker (NTAL), also known as linker for activation of B cells (LAB), is a transmembrane adaptor protein involved in immunoreceptor signaling. NTAL is expressed in lipid raft microdomains of B cells, mast cells, monocytes and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilon RI aggregation and Kit activation in mast cells, and Fc gamma RI aggregation in monocytes. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAT2
• Uniprot:
  • Q9GZY6()