NT5C2 Antibody

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in human cell line CAPAN-2.

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in control (vector only transfected HEK293T lysate) and NT5C2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Western Blot: NT5C2 Antibody [NBP1-85526] - Analysis in human cell line A-431 and human cell line HeLa.

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human testis shows cytoplasmic positivity in seminiferus duct cells.

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human liver shows weak to moderate cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: NT5C2 Antibody [NBP1-85526] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.

Product Discontinued

NT5C2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLA
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NT5C2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NT5C2 Antibody

• 5'-nucleotidase (purine), cytosolic type B
• 5'-nucleotidase, cytosolic II
• cN-II
• Cytosolic 5'-nucleotidase II
• EC 3.1.3
• EC 3.1.3.5
• GMP
• NT5BIMP-specific 5'-NT
• NT5CP
• PNT5cytosolic purine 5'-nucleotidase
• purine 5' nucleotidase

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NT5C2