Recombinant Human NT-3 Protein

SDS-Page: Recombinant Human NT-3 Protein [NBP3-18195] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Recombinant Human NT-3 Protein has a predicted molecular weight of 13.8 kDa when run under both reducing and non-reducing c

Product Discontinued

Recombinant Human NT-3 Protein Summary

• Description: An untagged bioactive recombinant protein corresponding to Human NT-3
  
  

  Source: E.coli

  Uniprot ID: P20783 / P20181

  Amino Acid Sequence: MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No Animal-Derived Ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin Levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: NT-3 is a dimer, 13.8/27.5kDa (120/240 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6), >5.0 x 10^4 units/mg (ED50 acceptance criteria are <20 ng/mL).
• Protein/Peptide Type: Recombinant Protein
• Gene: NTF3
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 micron filtered solution, 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support. Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.
Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied.
1 month when stored at 4C after reconstituting as directed.
3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human NT-3 Protein

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. Subcellular Location: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. Similarity: Belongs to the NGF-beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NTF3