Recombinant Human NT-3 Protein

Product Discontinued

Recombinant Human NT-3 Protein Summary

• Description:
  Tyr139-Thr257 (K196R)
  Accession # P20783
• Details of Functionality: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED_50 for this effect is 1-10 ng/mL. The specific activity of recombinant human NT-3 is approximately 2082
  U/ug, which is calibrated against Recombinant Human NT-3 Standard (NIBSC code: 98/718).
• Source: Sf 21 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: NTF3
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Endotoxin Note: <0.10 EU per 1 ug of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Reconstitution Instructions: Reconstitute 5 ug vials at 50 ug/mL in sterile PBS. Reconstitute 25 ug or larger vials at 100 ug/mL in sterile PBS.

Alternate Names for Recombinant Human NT-3 Protein

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer. The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NTF3