Recombinant Human NT-3 Protein

Product Discontinued

Recombinant Human NT-3 Protein Summary

• Description: Recombinant bioactive protein for Human Neurotrophin 3
  
  

  Source:E. coli
  Amino Acid Sequence:(P20783 )

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NTF3
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 13.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and then lyophilized with no additives
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human NT-3 Protein

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

NT-3 is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NT-3 is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. The molecular mass is 13.6 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NTF3