Human NT-3 ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
15.6 to 1000 pg/mL |
| Sensitivity |
2 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
NTF3 |
Applications/Dilutions
| Dilutions |
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| Application Notes |
NTF3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human NTF3. |
| Publications |
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Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 96-well plate precoated with anti-human Neurotrophin-3 antibody
- ABC diluent buffer
- Adhesive cover
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
- Biotinylated anti-human Neurotrophin-3 antibody, dilution 1:100
- Lyophilized recombinant human Neurotrophin-3 standard
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human NT-3 ELISA Kit (Colorimetric)
Background
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. Subcellular Location: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. Similarity: Belongs to the NGF-beta family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for NT-3 ELISA Kit (KA0402)(3)
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Product General Protocols
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FAQs for NT-3 ELISA Kit (KA0402). (Showing 1 - 1 of 1 FAQs).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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