NSFL1C Overexpression Lysate (Native)

Images

 
Western Blot: NSFL1C Overexpression Lysate (Adult Normal) [NBL1-13809] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NSFL1C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NSFL1C Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201022 and based on accession number NM_016143. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1, mRNA.
Gene
NSFL1C

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NSFL1C

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NSFL1C Overexpression Lysate (Native)

  • dJ776F14.1
  • NSFL1 (p97) cofactor (p47)
  • NSFL1 cofactor p47
  • p47
  • p97 cofactor p47
  • SHP1 homolog
  • UBX domain protein 2C
  • UBX domain-containing protein 2C
  • UBX1
  • UBXD10
  • UBXN2CMGC3347

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NSFL1C Lysate (NBL1-13809) (0)

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Bioinformatics

Gene Symbol NSFL1C