Rat Nrf2 ELISA Kit (Colorimetric)

ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Samples were spiked with high concentrations of Rat Nrf2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat Nrf2 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.99
• Intra-Assay: %CV < 5.08
• Spike Recovery: 87 - 104%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NFE2L2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Nrf2 ELISA Kit (Colorimetric)

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Nrf2 ELISA Kit (NBP3-08161)(7)

Publications using NBP3-08161

• Rania H. Abu-Baih, Dalia H. Abu-Baih, Sara Mohamed Naguib Abdel-Hafez, Moustafa Fathy Activation of SIRT1/Nrf2/HO-1 and Beclin-1/AMPK/mTOR autophagy pathways by eprosartan ameliorates testicular dysfunction induced by testicular torsion in rats Scientific Reports 2024-05-31 [PMID: 38822026]
• Dalia O. Saleh, Sara M. Baraka, Gehad A. Abdel Jaleel, Azza Hassan, Omar A. Ahmed-Farid Eugenol alleviates acrylamide-induced rat testicular toxicity by modulating AMPK/p-AKT/mTOR signaling pathway and blood–testis barrier remodeling Scientific Reports 2024-01-22 [PMID: 38253778]
• Mohamed F. Balaha, Ahmed A. Alamer, Rana M. Aldossari, Alhussain H. Aodah, Azza I. Helal, Ahmed M. Kabel, Patrick Geraghty Amentoflavone Mitigates Cyclophosphamide-Induced Pulmonary Toxicity: Involvement of -SIRT-1/Nrf2/Keap1 Axis, JAK-2/STAT-3 Signaling, and Apoptosis Medicina 2023-12-04 [PMID: 38138222]
• Jwharah M Al Jadani, Nawal A Albadr, Ghedeir M Alshammari, Soheir A Almasri, Farah Fayez Alfayez, Mohammed Abdo Yahya Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects. Nutrients 2023-11-27 [PMID: 38004149]
• ALTamimi JZ, AlFaris NA, Alshammari GM et al. Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2 Medicina (Kaunas, Lithuania) 2023-10-14 [PMID: 37893548] (ELISA)
• El-Deeb AM, Mohamed AF, El-Yamany MF, El-Tanbouly DM Novel trajectories of the NK1R antagonist aprepitant in rotenone-induced Parkinsonism-like symptoms in rats: Involvement of ERK5/KLF4/p62/Nrf2 signaling axis Chemico-biological interactions 2023-08-01 [PMID: 37224993] (ELISA)
• Ahmed M Kabel, Samir A Salama, Hany M Borg, Dina A Ali, Maaly A Abd Elmaaboud Targeting p-AKT/mTOR/MAP kinase signaling, NLRP3 inflammasome and apoptosis by fluvastatin with or without taxifolin mitigates gonadal dysfunction induced by bisphenol-A in male rats. Human & experimental toxicology 2022-04-26 [PMID: 35465754]

FAQs for Nrf2 ELISA Kit (NBP3-08161). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NFE2L2