Nrf2 Antibody (1B8) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (1B8) [H00004780-M03] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: Nrf2 Antibody (1B8) [H00004780-M03] - Detection limit for recombinant GST tagged NFE2L2 is approximately 0.1ng/ml as a capture antibody.

Western Blot-Nrf2 Antibody (1B8) [H00004780-M03] -Detection against Immunogen (36.63 KDa).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nrf2 Antibody (1B8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NFE2L2 (AAH11558, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVA
• Specificity: NFE2L2 - nuclear factor (erythroid-derived 2)-like 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NFE2L2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nrf2 Antibody (1B8) - Azide and BSA Free

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (H00004780-M03)(2)

Publications using H00004780-M03

• Ying-Jie Z, Hao Q, Dong-Fan L et al. Lithium promotes recovery after spinal cord injury. Neural Regen Res. 2021-11-12 [PMID: 34782578]
• Lindl KA, Jordan-Sciutto KL., et al. Examining the endogenous antioxidant response through immunofluorescent analysis of Nrf2 in tissue. Methods Mol Biol 2008-12-01 [PMID: 19082951]

Bioinformatics

• Gene Symbol: NFE2L2
• Entrez:
  • 4780(Human)
• OMIM:
  • 600492(Human)
• Uniprot:
  • AAH11558(Human)