NRAS Antibody

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Western Blot: NRAS Antibody [NBP2-19581] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: NRAS Antibody [NBP2-19581] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Western Blot: NRAS Antibody [NBP2-19581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with N-RAS antibody diluted at 1:5000. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NRAS Antibody Summary

Immunogen
Full length human N-Ras Recombinant protein.
Localization
Cell membrane; Lipid-anchor; Cytoplasmic side; Golgi apparatus membrane; Lipid-anchor
Predicted Species
Porcine (100%), Bovine (100%), Guinea Pig (99%), Zebrafish (90%), Chicken (96%), Canine (100%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NRAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NRAS Antibody

  • ALPS4
  • GTPase NRas
  • HRAS1
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • N-ras protein part 4
  • N-ras
  • NRAS1
  • NS6
  • Transforming protein N-Ras
  • v-ras neuroblastoma RAS viral oncogene homolog

Background

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NRAS Antibody (NBP2-19581). (Showing 1 - 1 of 1 FAQ).

  1. Can you tell me the Ig type of this antibody?
    • Rabbit polyclonal IgG

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NRAS
Uniprot