26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Striped and re-probed blot for actin as positive control. Furthermore, I have tested purified, recombinant NQO2 as a positive control (in a separate experiment not shown in the image file).Needed longer exposure times than for actin and B-tubulin controls (which were approximately 5' exposures).Aliquoted antibody and stored at -20 to make fresh stocks with each new blot, and worked well. A lot of washing with TBST removed excess background.
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