NQO-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-31563] - PLC xenograft, using NQO2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Sample (50 ug of whole cell lysate) A: Mouse liver 12% SDS PAGE NBP1-31563 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample(30 ug whole cell lysate)A:293TB:A431was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NQO-2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NQO-2 Antibody - BSA Free (NBP1-31563) is a polyclonal antibody validated for use in IHC and WB. Anti-NQO-2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NQO-2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NQO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raji Whole Cell Lysate
  Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
  HepG2 Whole Cell Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%), Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NQO-2 Antibody - BSA Free

• DHQV
• DIA6
• dioxin-inducible-2
• EC 1.10.99.2
• NAD(P)H dehydrogenase, quinone 2
• NAD(P)H menadione oxidoreductase 2, dioxin-inducible
• NMOR2
• NQO2
• NQO-2
• NRH dehydrogenase [quinone] 2
• NRH:quinone oxidoreductase 2
• QR2
• Quinone Reductase 2
• ribosyldihydronicotinamide dehydrogenase [quinone]
• ribosyldihydronicotinamide dehydrogenase

Background

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NQO-2 Antibody (NBP1-31563)(2)

Publications using NBP1-31563

• Miettinen TP, Bjorklund M. NQO2 Is a Reactive Oxygen Species Generating Off-Target for Acetaminophen. Mol Pharm 2014-12-01 [PMID: 25313982] (WB, Human)
• Roldan Urgoiti GB, GustafsoK, Klimowicz AC et al. The Prognostic Value of HPV Status and p16 Expression in Patients with Carcinoma of the Anal Canal. PLoS ONE 2014-10-02 [PMID: 25271758]

Review for NQO-2 Antibody (NBP1-31563) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer WB 08/21/2012

Summary

• Application: Western Blot
• Lot: 39946

Bioinformatics

• Gene Symbol: NQO2
• Entrez:
  • 4835(Human)
  • 18105(Mouse)
• Uniprot:
  • P16083(Human)