NQO-2 Antibody

Images

 
Western Blot: NQO-2 Antibody [NBP1-31563] - Sample(30 ug whole cell lysate)A:293T B:A431 12% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-31563] - Paraffin-embedded PLC xenograft, using antibody at 1:100 dilution.
Western Blot: NQO-2 Antibody [NBP1-31563] - Sample (50 ug of whole cell lysate) A: Mouse liver 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NQO-2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NQO2. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NQO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Raji Lysate (NB820-59466)
Liver Lysate (NB820-59662)
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31563 in the following applications:

Publications
Read Publications using
NBP1-31563 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (81%), Bovine (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NQO-2 Antibody

  • DHQV
  • DIA6
  • dioxin-inducible-2
  • EC 1.10.99.2
  • NAD(P)H dehydrogenase, quinone 2
  • NAD(P)H menadione oxidoreductase 2, dioxin-inducible
  • NMOR2
  • NQO2
  • NQO-2
  • NRH dehydrogenase [quinone] 2
  • NRH:quinone oxidoreductase 2
  • QR2
  • Quinone Reductase 2
  • ribosyldihydronicotinamide dehydrogenase [quinone]
  • ribosyldihydronicotinamide dehydrogenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NQO-2 Antibody (NBP1-31563)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Review for NQO-2 Antibody (NBP1-31563) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31563:
Filter by Applications
WB
(1)
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Filter by Species
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Images Ratings Applications Species Date Details
Western Blot NQO-2 NBP1-31563
Enlarge
reviewed by:
Anonymous
WB 08/21/2012
View

Summary

ApplicationWestern Blot
Lot39946
CommentsStriped and re-probed blot for actin as positive control. Furthermore, I have tested purified, recombinant NQO2 as a positive control (in a separate experiment not shown in the image file).Needed longer exposure times than for actin and B-tubulin controls (which were approximately 5' exposures).Aliquoted antibody and stored at -20 to make fresh stocks with each new blot, and worked well. A lot of washing with TBST removed excess background.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
08/21/2012
Application: WB
Species:

Bioinformatics

Gene Symbol NQO2
Entrez
Uniprot