NPY1R Antibody

Product Discontinued

NPY1R Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human NPY2-R.
• Specificity: NPY2-R (D42) detects endogenous levels of NPY2-R protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPY1R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500- 1:1000
• Application Notes: Western blot (WB) analysis of NPY2-R (D42) pAb in extracts from Hela cells.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NPY1R Antibody

• neuropeptide Y receptor type 1
• neuropeptide Y receptor Y1
• NPY1R
• NPY1-R
• NPYR
• NPYY1

Background

A gene on chromosome 4q31 encodes a 381 amino acid protein, NPY2-R(also designated Neuropeptide Y receptor Y2). NPY2-R is a member of the Gprotein-coupled receptor super-family, and like other members has seven putative transmembrane domains. However, NPY2-R gene consists of two exons, unlike the contiguous structure of other G-protein-coupled receptor genes. NPY2-R shares a 31% sequence identity with NPY1-R. NPY2-R is expressed in the presynaptic membranes of the central nervous system, and to a lesser extent the peripheral nervous system. NPY2-R associates (from highest to lowest affinity) with carboxyl terminals of unphosphorylate Peptide YY (PYY) (phosphorylation of PYY greatly reduces its binding affinity), NPY, and Pancreatic Peptide. Depending on the cell type, NPY2-R couples with different G proteins, which act as different second messengers. NPY2-R reduces the effects of depolarization, calcium ion currents in arterial smooth muscle by through association with NPY. NPY2-R inhibits cholecystokinin octapeptide-induced, esophageal muscle contraction, through interactions with PYY and NPY.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NPY1R Antibody (NBP1-00956). (Showing 1 - 1 of 1 FAQ).

1. Which cell lysate should I use with Anti-human Neuropeptide Y1R (cat# NBP1-00956)?
   • NPY1R: Kidney tubules, Stomach glandular cells, and Brain Cortex endothelial cells.

Bioinformatics

• Gene Symbol: NPY1R
• Entrez:
  • 4886(Human)
  • 66024(Rat)