NPM1 Antibody

Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NPM1 Antibody Summary

• Immunogen: between 100 and 150
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for NPM1 Antibody

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPM1