NPM1 Antibody (NPM1/3286) [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
NPM1/3286
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

NPM1 Antibody (NPM1/3286) [DyLight 650] Summary

Immunogen
Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
Localization
Nuclear, Cytoplasmic
Specificity
This antibody recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM1). It is predominantly localized in the nucleus of cells in most tissues. NPM1 is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NPM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NPM1 Antibody (NPM1/3286) [DyLight 650]

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NPM1 Antibody (NBP2-79875C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-79875AF350
Alexa Fluor 405 NBP2-79875AF405
Alexa Fluor 488 NBP2-79875AF488
Alexa Fluor 532 NBP2-79875AF532
Alexa Fluor 594 NBP2-79875AF594
Alexa Fluor 647 NBP2-79875AF647
Alexa Fluor 700 NBP2-79875AF700
Alexa Fluor 750 NBP2-79875AF750
Allophycocyanin NBP2-79875APC
Biotin NBP2-79875B
DyLight 350 NBP2-79875UV
DyLight 405 NBP2-79875V
DyLight 488 NBP2-79875G
DyLight 550 NBP2-79875R
DyLight 594 NBP2-79875DL594
DyLight 650 NBP2-79875C
DyLight 680 NBP2-79875FR
DyLight 755 NBP2-79875IR
FITC NBP2-79875F
HRP NBP2-79875H
Janelia Fluor 549 NBP2-79875JF549
Janelia Fluor 646 NBP2-79875JF646
PE NBP2-79875PE
PerCP NBP2-79875PCP

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Bioinformatics Tool for NPM1 Antibody (NBP2-79875C)

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Blogs on NPM1

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Bioinformatics

Gene Symbol NPM1