NPM1 Antibody (NPM1/3286)

Images

 
Western Blot: NPM1 Antibody (NPM1/3286) [NBP2-79740] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3286).
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (NPM1/3286) [NBP2-79740] - Immunofluorescence staining of K562 cells using NPM1 Antibody (NPM1/3286) followed by goat anti-Mouse IgG conjugated to CF488 (green). ...read more
Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3286) [NBP2-79740] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/3286).
Flow Cytometry: NPM1 Antibody (NPM1/3286) [NBP2-79740] - Flow Cytometric Analysis of A549 cells using NPM1 Antibody (NPM1/3286) (NPM1/3286); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Array: NPM1 Antibody (NPM1/3286) [NBP2-79740] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/3286) Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA
Clone
NPM1/3286
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

NPM1 Antibody (NPM1/3286) Summary

Immunogen
Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
Localization
Nuclear, Cytoplasmic
Specificity
This antibody recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM1). It is predominantly localized in the nucleus of cells in most tissues. NPM1 is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NPM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79875)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for NPM1 Antibody (NPM1/3286)

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPM1
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