NPM1 Antibody (NPM1/3286) - Azide and BSA Free

Western Blot: NPM1 Antibody (NPM1/3286) - Azide and BSA Free [NBP2-79875] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3286).

Immunocytochemistry/Immunofluorescence: NPM1 Antibody (NPM1/3286) - Azide and BSA Free [NBP2-79875] - Immunofluorescence staining of K562 cells using NPM1 Antibody (NPM1/3286) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with RedDot.

Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3286) - Azide and BSA Free [NBP2-79875] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/3286).

Flow Cytometry: NPM1 Antibody (NPM1/3286) - Azide and BSA Free [NBP2-79875] - Flow Cytometric Analysis of A549 cells using NPM1 Antibody (NPM1/3286) (NPM1/3286); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Protein Array: NPM1 Antibody (NPM1/3286) - Azide and BSA Free [NBP2-79875] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/3286) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, MA, CyTOF-ready
• Clone: NPM1/3286
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NPM1 Antibody (NPM1/3286) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79740).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary) (Uniprot: P06748)
• Localization: Nuclear, Cytoplasmic
• Marker: Acute Myeloid Leukemia Marker
• Specificity: Recognizes a 33kDa glycoprotein, identified as NPM1 (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for NPM1 Antibody (NPM1/3286) - Azide and BSA Free

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPM1