NPIP Antibody (39C7)

Immunocytochemistry/Immunofluorescence: NPIP Antibody (39C7) [NBP1-30038] - HeLa cells were stained with anti-nuclear pore complex antibody (green), and chicken anti-vimentin (red).

Product Discontinued

NPIP Antibody (39C7) Summary

• Immunogen: Yeast nuclear preparations
• Specificity: Reacts with a nuclear pore complex protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPIP
• Purity: Concentrated Supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
• Theoretical MW: 0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.07% Sodium Azide
• Purity: Concentrated Supernatant

Alternate Names for NPIP Antibody (39C7)

• FLJ42525
• FLJ44848
• nuclear pore complex interacting protein
• nuclear pore complex-interacting protein

Background

The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPIP