Human NOX1 ELISA Kit (Colorimetric)

ELISA: Human NOX1 ELISA Kit (Colorimetric) [NBP2-76746] - Samples were spiked with high concentrations of Human NOX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human NOX1 ELISA Kit (Colorimetric) [NBP2-76746] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL

Human NOX1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.78
• Intra-Assay: CV% < 4.79
• Spike Recovery: 85-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NOX1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human NOX1 ELISA Kit (Colorimetric)

• EC 1.6.3
• GP91-2
• Mitogenic oxidase 1
• MOX-1
• MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
• NADPH oxidase 1
• NADPH oxidase homolog-1
• NOH1
• NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
• NOH-1NADPH oxidase 1 variant NOH-1L
• NOX1
• NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for NOX1 ELISA Kit (NBP2-76746)(2)

Publications using NBP2-76746

• Kim, H;Suh, GJ;Kwon, WY;Kim, KS;Jung, YS;Kim, T;Park, H; Kallistatin deficiency exacerbates neuronal damage after cardiac arrest Scientific reports 2024-02-21 [PMID: 38383562] (ELISA)
• Chen X, Huang J, Yu C et al. A noncanonical function of EIF4E limits ALDH1B1 activity and increases susceptibility to ferroptosis Nature communications 2022-10-23 [PMID: 36274088] (ELISA)

FAQs for NOX1 ELISA Kit (NBP2-76746). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NOX1