NOX1 Antibody

Images

 
Western Blot: NOX1 Antibody [NBP1-69573] - NOX1 antibody - C-terminal region validated by WB using Epithelial Colorectal Adenocarcinoma CaCO2 at 1:10.
Western Blot: NOX1 Antibody [NBP1-69573] - This Anti-NOX1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 0.5ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOX1 Antibody Summary

Immunogen
Synthetic peptides corresponding to NOX1(NADPH oxidase 1) The peptide sequence was selected from the C terminal of human NOX1. Peptide sequence STIATSHPKSVVGVFLCGPRTLAKSLRKCCHRYSSLDPRKVQFYFNKENF.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
Application Notes
This is a rabbit polyclonal antibody against NOX1 and was validated on Western blot. Knockdown Validated and Use in and Immunocytochemistry/immunofluorescence and Immunohistochemistry-paraffin reported in scientific literature (PMID: 30096407).
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-69573 in the following applications:

  • 1 publication
  • 1 publication
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22683600). Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for NOX1 Antibody

  • EC 1.6.3
  • GP91-2
  • Mitogenic oxidase 1
  • MOX-1
  • MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
  • NADPH oxidase 1
  • NADPH oxidase homolog-1
  • NOH1
  • NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
  • NOH-1NADPH oxidase 1 variant NOH-1L
  • NOX1
  • NOX-1

Background

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox.Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. The NOH-1S currents were reversibly blocked by zinc, a known H+ channel inhibitor. The NOH-1S variant does not contain an electron transport chain and it is thought that H+ conductance is its main physiologic function, whereas NOH-1L may conduct H+ ions as part of its electron transport mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOX1 Antibody (NBP1-69573)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NOX1
Entrez
Uniprot